Wednesday, 11 March 2020, 09.30-18.00 h
- Welcome and organisational issues
- Basic knowledge identification of microorganisms
- Handling and examination of prepared cultures from different samples e.g. environmental monitoring, water on contact plates, swabs, membranes
- Preparation of pure cultures
- Introduction into the work with microscope and identification of microorganisms including staining procedures
- Practice of biochemical rapid tests
Thursday, 12 March 2020, 08.30-18.00 h
- Overview Molecular genetic methods and Mycoplasma detection
- Identification with Maldi-TOF MS and FTIR spectroscopy
- Practice in manual biochemical identification methods (Multi-Test-Systems, additional tests) and special media, e.g. chromogenic media using the prepared cultures
- Practice in molecular genetic rapid methods using the example of Mycoplasma detection
- Practice in identification of bacteria, yeasts and moulds by MALDI-TOF MS and FTIR spectroscopy
Friday, 13 March 2020, 08.00-15.30 h
- Analysis of the results from molecular genetic rapid methods, MALDI-TOF MS, FTIR spectroscopy and manual biochemical methods
- Increasing laboratory identification system performance with the use of quality control -Usage of reference strains
- Consolidation and discussion of all results from bacterial identification
- Basic knowledge and practice of yeast and moulds identification including microscopy
- Beneficial literature and helpful addresses