Course No 15191
If you have any questions, please contact us:
Tel.: +49 (0)6221 / 84 44 0 E-Mail: firstname.lastname@example.org
Barbara Gerten, Merck Millipore
Prof Dr Diana Graubaum, Beuth University of Applied Sciences
Alexander Bartes, Roche Diagnostics
Erika Tranfield, Bruker UK Ltd
Martina Held, Beuth University of Applied Sciences
Beate Volk, Beuth University of Applied Sciences
You will learn how to recognise microbiological cultures. You will further get to know classical procedures for the selection and identification and how to analyse the microbiological concept. In addition you will get an insight into the needed media and in modern rapid methods.
Due to the limited number of participants and the supervision by experienced lab staff you will be able to do the work, to employ the methods and to make the analyses yourself. For that purpose there will be lab places with microscopes in the S2 lab for every participant. Accompanying presentations and a comprehensive documentation will provide the necessary knowledge base.
Microbiological trainings are commonly based on presentations or theoretical workshops. Quite frequently there is either no advanced practical training at all or it is only very limited.
This completely new designed lab course is supposed to be an extension of the microbiological seminars as part of the GMP Certification Programme “Microbiological Laboratory Manager”.
Personnel from labs for
with basic microbiological knowledge.
A special thanks for supporting this course goes to VWR BioMérieux, Roche Diagnostics, Bruker, Dr. Weigert GmbH and Merck Millipore.
Wednesday, 9 March 2016, 10.00-18.00 h
Welcome and organisational issues
Basic knowledge Identification of
Introduction into the work with microscope and identification of microorganisms
Handling and examination of prepared cultures (different samples e.g. environmental monitoring, water on contact plates, swabs, membranes)
Preparation of pure cultures
Introduction in microscopy
Gram and spore staining
Biochemical rapid tests
Thursday, 10 March 2016, 09.00-17.30 h
Overview Molecular genetic methods and Mycoplasma detection
Identification with Maldi-TOF MS
Practice in manual biochemical identification methods (Multi-Test-Systems, additional tests)
Practice in molecular genetic rapid methods using the example of Mycoplasma detection
Practice in identification of bacteria, yeast and moulds by MALDI-TOF MS
Friday, 11 March 2016, 08.30-15.30 h
Analysis of the results from molecular genetic rapid methods, MALDI-TOF MS and manual biochemical methods
Consolidation and discussion of all results
Basic knowledge and practice of yeast and moulds identification including microscopy